关键词: 华蟾蜍毒素;肝肿瘤;白血病;细胞;比色法;氮蓝四唑;流式细胞术;显微镜检查，电子，扫描透射

    摘 要:目的 了解中药成分华蟾毒精(cinobufagin，CBG)的体外抗癌活性及其主要作用机制. 方法 采用流式细胞术检测华蟾毒精对体外培养的人肝癌细胞株SMMC-7721、人白血病细胞株HL-60的细胞毒作用;经透射电子显微镜观察CBG作用后培养的SMMC-7721及HL-60细胞超微结构的改变. 结果 华蟾毒精有显著的抗癌活性;对HL-60细胞的IC50 为1.25μg·L-1 ，对SMMC-7721细胞的IC50 为2.72μg·L-1 ，能非常显著地减少HL-60，SMMC-7721细胞S期DNA含量及增值指数，诱导癌细胞凋亡;透射电镜观察显示:华蟾毒精作用后，可见成片的细胞坏死，细胞凋亡，内质网肿胀、线粒体肿大呈空泡样，溶酶体增多等细胞结构改变. 结论 华蟾毒精有确切的抗癌作用，其作用产生的机制是多方面的，既能抑制癌细胞DNA的生物合成，又能诱导细胞凋亡.

    Experimental study on anticancer action of cinob┐ufagin in vitro

    ZHAO Jian-Bin CUI Qin ZHANG Xue WANG Wen-Ling JIANG Yong-Pei

    1 Department of Pharmacy，Xijing Hospital，

    2 Department of Cardiovascular Surgery，Xijing Hos-pital，

    3 Science Pharmaceutical Factory，

    4 Depart-ment of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:cinobufagin;liver neoplasms;leukemia;cells;colorimetry;nitroblue tetrazolium;flow cytome-try;microscopy，electron，scanning transmission

    Abstract:AIM To explore anticancer activity and mecha-nism of Cinobufagin(CBG)in vitro.METHODS Effect of CBG on the DNA content in S phase of cell cycle and the pro-liferative index of human hepatocarcionoma cell strain SMMC-7721and human leukemia cline HL-60cultuerd in vit-ro and the effect on inducing the cancer cell to apoptosis were checked up by flow cytometry the cytotoxic reaction of CBG on SMMC-7721and HL-60were investgated by the method of MTT.And the changes of the cellar ultrastructure induced by CBG were observed by the transmission electron mi┐croscopy.RESRLTS CBG had the remarking anticancer ac-tivity.The IC50 of CBG on HL-60was1.25μg·L-1 and the IC50 on SMMC-7721was2.72μg·L-1 .And the DNA con-tent and the proliferative index of HL-60SMMC-7721were evidently reduced.The cancer cell was induced to apoptosis by CBG.The changes of organelle structure ......